Bioeconomy deals with both sustainability and circularity and covers all biological resources. The aim of this Recommendation is to provide cities with a framework for bioeconomy’s development, especially under the lens of circularity and sustainability. The main elements examined in this document are: The definition and role of bioeconomy in cities, with a focus on circularity and sustainability. The determination of factors and KPIs that affect bioeconomy development in cities. The definition of a generic implementation framework for bioeconomy in cities.